Triterpenes from the Aerial Parts of Cimicifuga yunnanensis and Their Antiproliferative Effects on p53N236S Mouse Embryonic Fibroblasts

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• 作者：Yin Nian ; Hui Zhu ; Wen-Ru Tang ; Yin Luo ; Jiang ; Du ; Ming-Hua Qiu
• 刊名：Journal of Natural Products
• 出版年：2013
• 出版时间：May 24, 2013
• 年：2013
• 卷：76
• 期：5
• 页码：896-902
• 全文大小：319K
• 年卷期：v.76,no.5(May 24, 2013)
• ISSN：1520-6025

文摘

Nine new triterpene derivatives, yunnanterpenes A鈥揊 (1鈥?b>6), 15,16-seco-cimiterpenes A and B (7, 8), and cimilactone C (9), and 15 known analogues (10鈥?b>24) were isolated from the aerial parts of Cimicifuga yunnanensis. The new structures were established using a combination of MS, NMR, and single-crystal X-ray diffraction techniques. WT MEFs (wild-type mouse embryonic fibroblasts) and tumorigenic cell lines p53^鈥?鈥?/i>+H-RasV12 and p53^鈥?鈥?/i>+p53^N236S+H-RasV12 were used for evaluating active structures, targeting p53^N236S (corresponding to p53^N239S in humans) mutation. Compound 5 showed nonselective activities against these cell lines, with IC₅₀ values of 5.8, 8.6, and 6.0 渭M, respectively. Compound 4 exhibited greater selectivity against the p53^鈥?鈥?/i>+p53^N236S+H-RasV12 cells (IC₅₀ 5.5 渭M) than against the WT MEFs cells (IC₅₀ 14.3 渭M).