Scalable Production of Molybdenum Disulfide Based Biosensors

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• 作者：Carl H. Naylor ; Nicholas J. Kybert ; Camilla Schneier ; Jin Xi ; Gabriela Romero ; Jeffery G. Saven ; Renyu Liu ; A. T. Charlie Johnson
• 刊名：ACS Nano
• 出版年：2016
• 出版时间：June 28, 2016
• 年：2016
• 卷：10
• 期：6
• 页码：6173-6179
• 全文大小：502K
• 年卷期：0
• ISSN：1936-086X

文摘

We demonstrate arrays of opioid biosensors based on chemical vapor deposition grown molybdenum disulfide (MoS₂) field effect transistors (FETs) coupled to a computationally redesigned, water-soluble variant of the μ-opioid receptor (MOR). By transferring dense films of monolayer MoS₂ crystals onto prefabricated electrode arrays, we obtain high-quality FETs with clean surfaces that allow for reproducible protein attachment. The fabrication yield of MoS₂ FETs and biosensors exceeds 95%, with an average mobility of 2.0 cm² V^–1 s^–1 (36 cm² V^–1 s^–1) at room temperature under ambient (in vacuo). An atomic length nickel-mediated linker chemistry enables target binding events that occur very close to the MoS₂ surface to maximize sensitivity. The biosensor response calibration curve for a synthetic opioid peptide known to bind to the wild-type MOR indicates binding affinity that matches values determined using traditional techniques and a limit of detection ∼3 nM (1.5 ng/mL). The combination of scalable array fabrication and rapid, precise binding readout enabled by the MoS₂ transistor offers the prospect of a solid-state drug testing platform for rapid readout of the interactions between novel drugs and their intended protein targets.