Evidence for a Quasi-Equilibrium Distribution of States for Bradykinin [M + 3H]3+ Ions in the Gas Phase

详细信息    查看全文

• 作者：Nicholas A. Pierson ; Stephen J. Valentine ; David E. Clemmer
• 刊名：Journal of Physical Chemistry B
• 出版年：2010
• 出版时间：June 17, 2010
• 年：2010
• 卷：114
• 期：23
• 页码：7777-7783
• 全文大小：226K
• 年卷期：v.114,no.23(June 17, 2010)
• ISSN：1520-5207

文摘

Multidimensional ion mobility spectrometry coupled with mass spectrometry (IMS−IMS−MS) techniques are used to select and activate six different gas-phase conformations of bradykinin [M + 3H]³⁺ ions. Drift time distributions as a function of activation voltage show that at low voltages selected structures undergo conformational transitions in what appears to be a pathway dependent fashion. Over a relatively wide range of intermediate activation voltages a distribution of states that is independent of the initial conformation selected for activation (as well as the activation voltage in this intermediate region) is established. This distribution appears to represent an equilibrium distribution of gas-phase structures that is reached prior to the energy required for dissociation. Establishment of a quasi-equilibrium prior to dissociation results in identical dissociation patterns for different selected conformations. A discussion of the transition from solution-like to gas-phase structures is provided.