Nanoscale Patterning in Mixed Fluorocarbon-Hydrocarbon Phospholipid Bilayers
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- 作者：Nicholas C. Yoder ; Venkateshwarlu Kalsani ; Steffen Schuy ; Reiner Vogel ; Andreas Janshoff ; Krishna Kumar
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：July 25, 2007
- 年：2007
- 卷：129
- 期：29
- 页码：9037 - 9043
- 全文大小：751K
- 年卷期：v.129,no.29(July 25, 2007)
- ISSN：1520-5126
文摘
A growing body of literature suggests that fluorocarbons can direct self-assembly withinhydrocarbon environments. We report here the fabrication and characterization of supported lipid bilayers(SLBs) composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and a synthetic, fluorocarbon-functionalized analogue, 1. AFM investigation of these model membranes reveals an intricate, composition-dependent domain structure consisting of ~50 nm stripes interspersed between ~1 
m sized domains.Although DSC of 1 showed a phase transition near room temperature, DSC of DPPC:1 mixtures exhibitedcomplex phase behavior suggesting domain segregation. Finally, temperature-dependent AFM of DPPC:1bilayers shows that, while the stripe structures can be melted above the Tm of 1, the stripes and domainsresult from immiscibility of the hydrocarbon and fluorocarbon lipid gel phases. Fluorination appears to bea promising strategy for chemical self-assembly in two dimensions. In particular, because no modificationis made to the lipid headgroups, it may be useful for nanopatterning biologically relevant ligands on bilayersin vitro or in living cells.
m sized domains.Although DSC of 1 showed a phase transition near room temperature, DSC of DPPC:1 mixtures exhibitedcomplex phase behavior suggesting domain segregation. Finally, temperature-dependent AFM of DPPC:1bilayers shows that, while the stripe structures can be melted above the Tm of 1, the stripes and domainsresult from immiscibility of the hydrocarbon and fluorocarbon lipid gel phases. Fluorination appears to bea promising strategy for chemical self-assembly in two dimensions. In particular, because no modificationis made to the lipid headgroups, it may be useful for nanopatterning biologically relevant ligands on bilayersin vitro or in living cells.