Spherical Nanoparticle Supported Lipid Bilayers for the Structural Study of Membrane Geometry-Sensitive Molecules
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- 作者：Riqiang Fu ; Richard L. Gill Jr. ; Edward Y. Kim ; Nicole E. Briley ; Erin R. Tyndall ; Jie Xu ; Conggang Li ; Kumaran S. Ramamurthi ; John M. Flanagan ; Fang Tian
- 刊名：Journal of the American Chemical Society
- 出版年：2015
- 出版时间：November 11, 2015
- 年：2015
- 卷：137
- 期：44
- 页码：14031-14034
- 全文大小：277K
- ISSN：1520-5126
文摘
Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural and topological studies of membrane geometry-sensitive molecules.