The separation of magnetic microparticles was achievedby on-chip free-flow magnetophoresis. In continuous flow,magnetic particles were deflected from the direction oflaminar flow by a perpendicular magnetic field dependingon their magnetic susceptibility and size and on the flowrate. Magnetic particles could thus be separated from eachother and from nonmagnetic materials. Magnetic andnonmagnetic particles were introduced into a microfluidicseparation chamber, and their deflection was studiedunder the microscope. The magnetic particles were 2.0and 4.5
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m in diameter with magnetic susceptibilities of1.12 × 10
-4 and 1.6 × 10
-4 m
3 kg
-1, respectively. The4.5-
![](/images/entities/mgr.gif)
m particles with the larger susceptibility were deflected further from the direction of laminar flow than the2.0-
![](/images/entities/mgr.gif)
m magnetic particles. Nonmagnetic 6-
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m polystyrene beads, however, were not deflected at all. Furthermore, agglomerates of magnetic particles were found tobe deflected to a larger extent than single magneticparticles. The applied flow rate and the strength andgradient of the applied magnetic field were the keyparameters in controlling the deflection. This separationmethod has a wide applicability since magnetic particlesare commonly used in bioanalysis as a solid supportmaterial for antigens, antibodies, DNA, and even cells.Free-flow magnetophoretic separations could be hyphenated with other microfluidic devices for reaction andanalysis steps to form a micro total analysis system.