Hydrolytic Enzymes as (Bio)-Logic for Wireless and Chipless Biosensors

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• 作者：Nigel F. Reuel ; Joseph C. McAuliffe ; Gregory A. Becht ; Mehrdad Mehdizadeh ; Jeffrey W. Munos ; RuPing Wang ; William J. Delaney
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：April 22, 2016
• 年：2016
• 卷：1
• 期：4
• 页码：348-353
• 全文大小：621K
• ISSN：2379-3694

文摘

The switchable activity of allosteric, hydrolytic enzymes was used as a single-input, “buffer” logic gate (performing YES and NOT) in a screen-printable biosensor. The enzyme substrate functioned as an “AND” logic gate with the enzyme and cofactor as inputs. These (bio)-logic materials transduced a signal by the cofactor activating the enzyme which then degraded the substrate that formed the dielectric of a tuning capacitor in an inductor-capacitor (LC) circuit. The degradation of the substrate was engineered to shift the capacitance and thus the resonant frequency of the device. The resonant frequency was monitored wirelessly with a low-power vector network analyzer observing the S21 parameter. Proof of concept was shown with subtilisin as the enzyme, activated by calcium (100 μg/mL and 5 mM, respectively) degrading a collagen substrate with a demonstrated wireless read range of up to 4 cm. Selectivity over other divalent cations (magnesium, copper II, and manganese II) and the effect of receiver motion were also shown on the wireless measurement.