A Comprehensive Compositional Analysis of Heparin/Heparan Sulfate-Derived Disaccharides from Human Serum

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• 作者：Wei Wei ; Milady R. Nin虄onuevo ; Anish Sharma ; Lieza M. Danan-Leon ; Julie A. Leary
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：May 15, 2011
• 年：2011
• 卷：83
• 期：10
• 页码：3703-3708
• 全文大小：792K
• 年卷期：v.83,no.10(May 15, 2011)
• ISSN：1520-6882

文摘

The analysis of heparan sulfate glycosaminoglycans (HSGAGs) variations in human serum at the disaccharide level has a great potential for disease diagnosis and prognosis. However, the lack of available analytical methodology for the compositional analysis of HSGAGs in human serum remains to be addressed to delineate the possible role of HSGAGs on the onset and/or progression of a disease. In this study, we have developed a method for the in-depth compositional analysis of the 12 heparin/HS-derived disaccharides from human serum using a combination of technologies鈥攆ractionation, exhaustive digestion, solid phase extraction, and LC鈥揗S/MS. The method exhibits high recovery (72鈥?10%) and good reproducibility (standard deviation of less than 5%) with a low limit of detection and quantification. Errors from the method validation were within 1.1%. Nondetectable non- or low-sulfated disaccharides in human serum were also detected using the optimized protocol. Further applying this method, the comprehensive analysis of HSGAGs compositions in human sera from female donors showed considerable variations in disaccharide patterns and compositions.