Fasciola hepatica, a trematode helminth, causes an economicall
y important disease (fasciolosis) in ruminants worldwide. Proteomic anal
ysis of the parasite provides valuable information to understand the relationship between the parasite and its host. Previous studies have identified various parasite proteins, some of which are considered as vaccine candidates or important drug targets. However, the approximate distribution and abundance of the proteins on the surface and within internal parts of the liver fluke are unknown. In this stud
y, two fractions including surface protein fraction (representing surface part of the parasite, near subplasma membrane of the tegument and above the basal membrane of the tegument) and internal protein fraction (representing internal part of the parasite, mainl
y deeper sides of the tegument including subbasal membrane and other further internal elements of the parasite) were obtained. Components of these two fractions were investigated b
y an advanced proteomics approach using a high-definition mass spectrometer with nano electrospra
y ionization source coupled to a high-performance liquid chromatograph
y s
ystem (na
noUPLC鈥揈SI鈥搎TOF鈥揗S). FABP1 was found highl
y abundant in the SPF fraction. Potentiall
y novel
F. hepatica proteins showing homolog
y with AKT interacting protein (
Xenopus tropicalis), sterol
O-ac
yltransferase 2 (
Homo sapiens), and integrin beta 7 (
Mus musculus) were identified with high quantities in onl
y the surface fraction of the parasite and ma
y be possible candidates for future control strategies.
Keywords:
Fasciola hepatica; surface protein fraction; label-free proteomics; yltransferase+2&qsSearchArea=searchText">sterol O-acyltransferase 2; integrin beta 7; AKT interacting protein