文摘
The inner walls of gold nanotubes, prepared by template synthesis in the nanopores of polycarbonate track etch membranes, have beenchemically modified with peptide nucleic acid (PNA) and used for label-free quantification of complementary DNA sequences. Selective bindingof DNA to the PNA-modified nanotubes is shown to decrease the flux of optically detected anionic markers through the nanotubes in aconcentration-dependent manner. The strong dependence of the biorecognition-modulated ion transport through the nanopores on the ionicstrength suggests a dominantly electrostatic exclusion mechanism of the ion flux decrease as a result of DNA binding to the PNA-modifiednanopores.