Production of L-lactic Acid and Oligomeric Compounds from Apple Pomace by Simultaneous Saccharification and Fermentation: A Response Surface Methodology Assessment
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- 作者：Beatriz Gulló ; n ; Gil Garrote ; José ; Luis Alonso ; J. C. Parajó
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：July 11, 2007
- 年：2007
- 卷：55
- 期：14
- 页码：5580 - 5587
- 全文大小：440K
- 年卷期：v.55,no.14(July 11, 2007)
- ISSN：1520-5118
文摘
The potential of apple pomace for lactic acid production by simultaneous saccharification andfermentation (SSF) was evaluated. The effects of the cellulase to solid ratio (CSR), the liquor to solidratio (LSR), and the 
-glucosidase to cellulase ratio (BCR) on the kinetics of lactic acid generationwere assessed, and a set of mathematical models was developed to reproduce and predict the lacticacid concentration of fermentation broths. Operating at low cellulase and cellobiase charges (1 FPU/gand 0.25 IU/FPU, respectively) and short reaction times (10 h), SSF media containing 27.8 g oflactic acid/L were obtained with a volumetric productivity of 2.78 g/Lh. Material balances showedthat the SSF processing of 100 kg of dry apple pomace results in the production of 36.6 kg of lacticacid, 18.3 kg of oligomeric carbohydrates (which can be used as ingredients for functional foods),8.4 kg of microbial biomass, and 8 kg uronic acids.
-glucosidase to cellulase ratio (BCR) on the kinetics of lactic acid generationwere assessed, and a set of mathematical models was developed to reproduce and predict the lacticacid concentration of fermentation broths. Operating at low cellulase and cellobiase charges (1 FPU/gand 0.25 IU/FPU, respectively) and short reaction times (10 h), SSF media containing 27.8 g oflactic acid/L were obtained with a volumetric productivity of 2.78 g/Lh. Material balances showedthat the SSF processing of 100 kg of dry apple pomace results in the production of 36.6 kg of lacticacid, 18.3 kg of oligomeric carbohydrates (which can be used as ingredients for functional foods),8.4 kg of microbial biomass, and 8 kg uronic acids.