Antioxidant Capacity of Plasma after Red Wine Intake in Human Volunteers
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- 作者：M. S. Ferná ; ndez-Pachó ; n ; D. Villañ ; o ; A. M. Troncoso ; and M. C. Garcí ; a-Parrilla
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：June 15, 2005
- 年：2005
- 卷：53
- 期：12
- 页码：5024 - 5029
- 全文大小：68K
- 年卷期：v.53,no.12(June 15, 2005)
- ISSN：1520-5118
文摘
Plasma antioxidant capacity (AC) has been assessed in eight healthy human volunteers after wineintake. Analytical methods used for evaluating AC included the ferric reducing ability of plasma (FRAP)and oxygen radical absorbance capacity using two different fluorescent probes, 
-phycoerythrin(ORAC-PE) and fluorescein (ORAC-FL). In addition, the concentrations of endogenous antioxidantssuch as uric acid, albumin, and bilirubin were determined. The suitability of analytical methods wasevaluated with two different biological matrixes: plasma and serum. Plasma AC was determinedbefore ingestion of 300 mL of red wine (baseline value) and 30, 55, and 120 min afterward. Maximumaverage increase in AC values was reached at 55 min. Plasma AC determined by ORAC-PE at timezero was significantly correlated with albumin concentration. Plasma AC determined with FRAP attime zero is well correlated with uric acid. Moreover, a good linear correlation was found betweenuric acid concentration and AC determined by FRAP in each volunteer. The maximum concentrationof uric acid occurred after 55 min. Uric acid increase accounts for a nonnegligible part in FRAP valuesand must be evaluated when using this method for assessing AC in plasma.Keywords: Antioxidant capacity; wine; plasma; serum; phenolic compounds; ORAC; phycoerythrin;fluorescein; FRAP; uric acid; human volunteers
-phycoerythrin(ORAC-PE) and fluorescein (ORAC-FL). In addition, the concentrations of endogenous antioxidantssuch as uric acid, albumin, and bilirubin were determined. The suitability of analytical methods wasevaluated with two different biological matrixes: plasma and serum. Plasma AC was determinedbefore ingestion of 300 mL of red wine (baseline value) and 30, 55, and 120 min afterward. Maximumaverage increase in AC values was reached at 55 min. Plasma AC determined by ORAC-PE at timezero was significantly correlated with albumin concentration. Plasma AC determined with FRAP attime zero is well correlated with uric acid. Moreover, a good linear correlation was found betweenuric acid concentration and AC determined by FRAP in each volunteer. The maximum concentrationof uric acid occurred after 55 min. Uric acid increase accounts for a nonnegligible part in FRAP valuesand must be evaluated when using this method for assessing AC in plasma.Keywords: Antioxidant capacity; wine; plasma; serum; phenolic compounds; ORAC; phycoerythrin;fluorescein; FRAP; uric acid; human volunteers