Coupling of Sequential Injection Chromatography with Multivariate Curve Resolution-Alternating Least-Squares for Enhancement of Peak Capacity

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• 作者：Ver&oacute ; nica G&oacute ; mez ; Manuel Mir&oacute ; Marí ; a Pilar Callao ; Ví ; ctor Cerd&agrave
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：October 15, 2007
• 年：2007
• 卷：79
• 期：20
• 页码：7767 - 7774
• 全文大小：336K
• 年卷期：v.79,no.20(October 15, 2007)
• ISSN：1520-6882

文摘

Flow-through low-pressure chromatographic separationscapitalized on the sequential injection chromatographic(SIC) concept are for the first time coupled to second-order multivariate regression models based on multivariate curve resolution-alternating least-squares (MCR-ALS)for outperforming current chromatographic methods interms of resolution efficiency. The proposed SIC-MCR-ALS method involving sequential injection separation onshort monolithic columns along with isocratic elutionfosters ultrafast reversed-phase separations of complexmulticomponent mixtures regardless of peak overlappingand retention parameters. The ruggedness of SIC systemsis enhanced by removing the solenoid valves from the flownetwork, thus diminishing the column back pressureeffects. As a consequence, the flow setup admitted mobile-phase flow rates much higher than those traditionallyenabled in SIC. To ascertain the improved peak capacityof the SIC-MCR-ALS procedure, five phenolic speciescommonly used in disinfectant products and featuringsimilar UV spectra and close retention times in shortreversed-phase silica-based monolithic phases are selected as model compounds and determined in just 1 minusing mobile-phase flow rates of 2 mL min^-1. Notwithstanding the fact that the five phenolic derivatives coelutein a single chromatographic band, thus rendering resolution values ranging from 0.05 to 1.11, the concentrationprofiles and the pure spectra of each individual phenolspecies could be concurrently obtained. Quantitativevalidation of the chromatographic-chemometric methoddemonstrated both the reliability of the results and theenhanced resolution of mixtures with regard to former SICsystems with no need for thorough optimization of theseparation conditions.