Interfacial Membrane Effects of Fluoroquinolones as Revealed by a Combination of Fluorescence Binding Experiments and Atomic Force Microscopy Observations
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- 作者：M. Teresa Montero ; Mò ; nica Pijoan ; Sandra Merino-Montero ; Teresa Vinuesa ; Jordi Herná ; ndez-Borrell
- 刊名：Langmuir
- 出版年：2006
- 出版时间：August 29, 2006
- 年：2006
- 卷：22
- 期：18
- 页码：7574 - 7578
- 全文大小：371K
- 年卷期：v.22,no.18(August 29, 2006)
- ISSN：1520-5827
文摘
6-Fluoroquinolones are useful antimicrobial agents against gram-positive and gram-negative bacteria and somemycobacterial species as well. Although the diffusion through porins in gram-negative bacteria is well established,other mechanisms such as the hydrophobic pathway through the apolar regions of the bilayer and the self-promotedpathway appear to be relevant or concomitant with the hydrophilic pathway in many cases. This article discusses theinteraction of ciprofloxacin (CPX) and two new synthesized compounds (M3CPX and M4CPX)-with a methyl groupattached at the N3 and N4 positions of the piperazynil ring of the CPX-with liposomes and supported planar bilayers(SPBs) of Escherichia coli. Binding experiments using ANS revealed that the three compounds interact electrostaticallywith the bilayer. The variations in the electrostatic surface potential, which is always positive, were higher for M3CPXthan for CPX or M4CPX. Related to that, the SPBs of E. coli were more affected by M3CPX than by the other twocompounds, as judged by the analysis of the atomic force microcopy (AFM) images. The in situ injection of the three6-fluoroquinolones (6-FQs) induced different changes in height, roughness (Ra), and area covered by the SPBs.