Antimutagenicity of Some Edible Thai Plants, and a Bioactive Carbazole Alkaloid, Mahanine, Isolated from Micromelum minutum
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- 作者：Kazuhiko Nakahara ; Gassinee Trakoontivakorn ; Najeeb S. Alzoreky ; Hiroshi Ono ; Mayumi Onishi-Kameyama ; and Mitsuru Yoshida
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：August 14, 2002
- 年：2002
- 卷：50
- 期：17
- 页码：4796 - 4802
- 全文大小：196K
- 年卷期：v.50,no.17(August 14, 2002)
- ISSN：1520-5118
文摘
The antimutagenic activity against Trp-P-1 of methanolic extracts of 118 samples (108 species) ofedible Thai plants was examined by the Ames Test. The activity was evaluated by the amount ofplant extracts which suppressed 90% of the mutagenesis (ED90). Five plants, Micromelum minutum,Oroxylum indicum, Cuscuta chinensis, Azadirachta indica, and Litsea petiolata, exhibited significantactivity with antimutagenic ED90 values lower than 5 
L/plate (0.1 mg of dry plant material equivalent).The activity-guided fractionation of the extract of M. minutum, which exhibited the highest antimutagenicactivity in the screening, resulted in the isolation of an active principle, (+)-mahanine (1) as confirmedby its physicochemical properties. Compound 1 showed a wide variety of biological activity, includingantimutagenicity against heterocyclic amines such as Trp-P-1 with an IC50 of 5.2 M, cytotoxicityagainst a tumor cell line HL60 with a MIC100 of 4.0 g/mL, and antimicrobial activity against Bacilluscereus and Staphylococcus aureus with MIC100 values of 6.25 and 12.5 g/mL, respectively.Keywords: Antimutagen; Trp-P-1; Micromelum minutum; mahanine
L/plate (0.1 mg of dry plant material equivalent).The activity-guided fractionation of the extract of M. minutum, which exhibited the highest antimutagenicactivity in the screening, resulted in the isolation of an active principle, (+)-mahanine (1) as confirmedby its physicochemical properties. Compound 1 showed a wide variety of biological activity, includingantimutagenicity against heterocyclic amines such as Trp-P-1 with an IC50 of 5.2 M, cytotoxicityagainst a tumor cell line HL60 with a MIC100 of 4.0 g/mL, and antimicrobial activity against Bacilluscereus and Staphylococcus aureus with MIC100 values of 6.25 and 12.5 g/mL, respectively.Keywords: Antimutagen; Trp-P-1; Micromelum minutum; mahanine