Identification of Metallothionein Subisoforms in HPLC Using Accurate Mass and Online Sequencing by Electrospray Hybrid Linear Ion Trap-Orbital Ion Trap Mass Spectrometry

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• 作者：Sandra Mounicou ; Laurent Ouerdane ; BatriceL’ ; Azou ; Isabelle Passagne ; ClineOhayon-Courts ; Joanna Szpunar ; Ryszard Lobinski
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：August 15, 2010
• 年：2010
• 卷：82
• 期：16
• 页码：6947-6957
• 全文大小：386K
• 年卷期：v.82,no.16(August 15, 2010)
• ISSN：1520-6882

文摘

A comprehensive approach to the characterization of metallothionein (MT) isoforms based on microbore HPLC with multimodal detection was developed. MTs were separated as Cd₇ complexes, detected by ICP MS and tentatively identified by molecular mass measured with 1−2 ppm accuracy using Orbital ion trap mass spectrometry. The identification was validated by accurate mass of the corresponding apo-MTs after postcolumn acidification and by their sequences acquired online by higher-energy collision dissociation MS/MS. The detection limits down to 10 fmol and 45 fmol could be obtained by ESI MS for apo- and Cd₇-isoforms, respectively, and were lower than those obtained by ICP MS (100 fmol). The individual MT isoforms could be sequenced at levels as low as 200 fmol with the sequence coverage exceeding 90%. The approach was successfully applied to the identification of MT isoforms induced in a pig kidney cell line (LLC-PK₁) exposed to CdS nanoparticles.