Adsorption and Self-Assembly of Oligodeoxynucleotides onto a Mica Surface

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• 作者：Zoltá ; n Bá ; lint ; Krisztina Nagy ; Ilona Laczkó ; Sá ; ndor Bottka ; Gergely A. Vé ; gh ; Zsolt Szegletes ; Gy&ouml ; rgy Vá ; ró
• 刊名：Journal of Physical Chemistry C
• 出版年：2007
• 出版时间：November 15, 2007
• 年：2007
• 卷：111
• 期：45
• 页码：17032 - 17037
• 全文大小：837K
• 年卷期：v.111,no.45(November 15, 2007)
• ISSN：1932-7455

文摘

Atomic force microscopy has been used to study the specific structures of oligodeoxynucleotides (ODN)dried onto a freshly cleaved muscovite mica surface. The nucleotides have an important role in biology asthe building blocks of the information coding DNA. To fulfill this task, the nucleotides must have specificself-assembling properties. The appearance of self-assembly was monitored as a function of different externalfactors, such as the concentration of the ODN, pH of the solution, and the composition of the surface material.At low ODN concentrations, around 20 nM, fiber-like formations appeared. At a rather high ODN concentration,around 400 nM, a network-like structure was seen. These formations appear only when the pH of the solutionfalls to between 3.3 and 4. Several surfaces were investigated, but the self-assembly of the ODN was observedonly in the case of mica surfaces.