An iTRAQ-Based Quantitative Analysis To Elaborate the Proteomic Response of Nostoc sp. PCC 7120 under N2 Fixing Conditions

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• 作者：Karin Stensj&ouml ; Saw Yen Ow ; Martin E. Barrios-Llerena ; Peter Lindblad ; Phillip C. Wright
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：February 2007
• 年：2007
• 卷：6
• 期：2
• 页码：621 - 635
• 全文大小：606K
• 年卷期：v.6,no.2(February 2007)
• ISSN：1535-3907

文摘

Nostoc sp. PCC 7120 is an oxygen-evolving photoautotrophic N₂ fixing filamentous cyanobacterium.Upon nitrogen starvation, a range of processes are initiated, such as differentiation of the heterocysts,specific cells where N₂ fixation takes place. We have characterized and quantified the proteome of theNostoc sp. PCC 7120 wild-type strain grown under N₂ fixing and non-N₂ fixing conditions. To assessglobal proteome changes in response to environmental changes, measurements were made using thequantitative proteomics tool, iTRAQ, on a whole cell digest. From this approach, a total of 486 differentproteins was accurately identified across 2 biological replicate experiments, where 226 identificationscontained 2 or more distinct peptides. Results of metabolic regulation will be discussed to demonstratethat proteomics represents an important tool for the development of heterocystous cyanobacteria forfuture biological H₂ production.