文摘
The anti-inflammatory properties of phenolic pine (Pinus sylvestris L.) bark extract were studied. Thepine bark extract was fractionated by liquid-liquid extractions and semipreparative high-performanceliquid chromatography to reveal the most potent constituents. The phenolic compositions of threepine bark samples obtained, a crude extract, a chloroform fraction, and a semipreparative fraction,were analyzed using high-performance liquid chromatography with UV diode array detection and/orelectrospray ionization mass spectrometry. In addition, eight compounds were isolated and identifiedby NMR and MS techniques. In total 28 phenolic compounds were identified. The effects of the threepine bark samples on the synthesis of two proinflammatory mediators, nitric oxide and prostaglandinE2, were measured. It was shown that pine bark contains compounds that inhibit the production ofthese proinflammatory mediators.Keywords: Bark; HPLC-DAD; HPLC-ESI-MS; inflammation; macrophages; nitric oxide; nitric oxidesynthase; phenolic compounds; Pinus sylvestris L.