Molecular Response of Crop Plants to Engineered Nanomaterials

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• 作者：Luca Pagano ; Alia D. Servin ; Roberto De La Torre-Roche ; Arnab Mukherjee ; Sanghamitra Majumdar ; Joseph Hawthorne ; Marta Marmiroli ; Elena Maestri ; Robert E. Marra ; Susan M. Isch ; Om Parkash Dhankher ; Jason C. White ; Nelson Marmiroli
• 刊名：Environmental Science & Technology
• 出版年：2016
• 出版时间：July 5, 2016
• 年：2016
• 卷：50
• 期：13
• 页码：7198-7207
• 全文大小：549K
• 年卷期：0
• ISSN：1520-5851

文摘

Functional toxicology has enabled the identification of genes involved in conferring tolerance and sensitivity to engineered nanomaterial (ENM) exposure in the model plant Arabidopsis thaliana (L.) Heynh. Several genes were found to be involved in metabolic functions, stress response, transport, protein synthesis, and DNA repair. Consequently, analysis of physiological parameters, metal content (through ICP-MS quantification), and gene expression (by RT-qPCR) of A. thaliana orthologue genes were performed across different plant species of agronomic interest to highlight putative biomarkers of exposure and effect related to ENMs. This approach led to the identification of molecular markers in Solanum lycopersicum L. and Cucurbita pepo L. (tomato and zucchini) that might not only indicate exposure to ENMs (CuO, CeO₂, and La₂O₃) but also provide mechanistic insight into response to these materials. Through Gene Ontology (GO) analysis, the target genes were mapped in complex interatomic networks representing molecular pathways, cellular components, and biological processes involved in ENM response. The transcriptional response of 38 (out of 204) candidate genes studied varied according to particle type, size, and plant species. Importantly, some of the genes studied showed potential as biomarkers of ENM exposure and effect and may be useful for risk assessment in foods and in the environment.