Application of Affinity Selection/Mass Spectrometry to Determine the Structural Isomer of Parnafungins Responsible for Binding Polyadenosine Polymerase

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• 作者：Gregory C. Adam ; Craig A. Parish ; Douglas Wisniewski ; Juncai Meng ; Min Liu ; Kathleen Calati ; Benjamin D. Stein ; John Athanasopoulos ; Paul Liberator ; Terry Roemer ; Guy Harris ; Kevin T. Chapman
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：December 10, 2008
• 年：2008
• 卷：130
• 期：49
• 页码：16704-16710
• 全文大小：389K
• 年卷期：v.130,no.49(December 10, 2008)
• ISSN：1520-5126

文摘

To discover antifungal treatments that possess the desired characteristics of broad spectrum activity, a strong safety profile, and oral bioavailability, new discovery strategies must be implemented to identify structural classes of molecules capable of combating these microorganisms. One such technique that has been implemented is the Candida albicans Fitness Test, a whole cell screening platform capable of delineating the mechanism of action of compounds that demonstrate activity against the clinically relevant pathogenic fungus, C. albicans. Screening crude natural product extracts with this technology has resulted in the identification of a novel family of antifungal natural products, named the parnafungins, which inhibit the enzyme polyadenosine polymerase (PAP), a key component of the mRNA cleavage and polyadenylation complex. Owing to the rapid interconversion of the structural and stereoisomers of the parnafungins at neutral pH, the determination of the structural isomer with the highest affinity for PAP with standard biochemical assays has not been possible. Herein, we present an application of affinity-selection/mass spectrometry (AS−MS) to determine that the “straight” parnafungin structural isomer (parnafungin A) binds preferentially to PAP compared to the “bent” structural isomer (parnafungin B).