Effectiveness of Microsatellite DNA Markers in Checking the Identity of Protected Designation of Origin Extra Virgin Olive Oil
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文摘
Collina di Brindisi is an Italian extra virgin olive oil that obtained the mark of protected designation oforigin (PDO) according to EC Regulation 2081/92. The varietal requirements of the official productionprotocol of this oil foresee that this oil is prepared from cultivar Ogliarola (minimum 70%) and otherOlea europaea L. cultivars that are diffused in the production area, accounting for a maximum of30%. The aim of this work was to verify the effectiveness of microsatellite analysis in verifying theidentity of Collina di Brindisi PDO olive oil. A preliminary assessment of product's quality by meansof chemical analyses was also carried out. Microsatellite analysis clarified that the generic nameOgliarola, indicated in the technical sheet of this PDO oil, actually corresponded to the Ogliarolasalentina cultivar. Furthermore, the obtained results showed that the examination of a limited numberof DNA microsatellites enables the identification of the Ogliarola salentina cultivar in this PDO oil.

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