Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity
详细信息 查看全文
- 作者：Andrii Rogov ; Marie Irondelle ; Fernanda Ramos Gomes ; Julia Bode ; Davide Staedler ; Sol猫ne Passemard ; S茅bastien Courvoisier ; Yasuaki Yamamoto ; Fran莽ois Waharte ; Daniel Ciepielewski ; Philippe Rideau ; Sandrine Gerber-Lemaire ; Frauke Alves ; Jean Salamero ; Luigi Bonacina ; Jean-Pierre Wolf
- 刊名：ACS Photonics
- 出版年：2015
- 出版时间：October 21, 2015
- 年：2015
- 卷：2
- 期：10
- 页码：1416-1422
- 全文大小：573K
- ISSN：2330-4022
文摘
We investigate the use of bismuth ferrite (BFO) nanoparticles for tumor tissue labeling in combination with infrared multiphoton excitation at 1250 nm. We report the efficient and simultaneous generation of second- and third-harmonic signals by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light鈥揺lectron microscopy.