Identification of Pectenotoxin-11 as 34S-Hydroxypectenotoxin-2, a New Pectenotoxin Analogue in the Toxic Dinoflagellate Dinophysis acuta from New Zealand
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- 作者：Toshiyuki Suzuki ; John A. Walter ; Patricia LeBlanc ; Shawna MacKinnon ; Christopher O. Miles ; Alistair L. Wilkins ; Rex Munday ; Veronica Beuzenberg ; O ; A. Lincoln MacKenzie ; O ; Dwayne
- 刊名：Chemical Research in Toxicology
- 出版年：2006
- 出版时间：February 2006
- 年：2006
- 卷：19
- 期：2
- 页码：310 - 318
- 全文大小：213K
- 年卷期：v.19,no.2(February 2006)
- ISSN：1520-5010
文摘
A new pectenotoxin, which has been named pectenotoxin-11 (PTX11), was isolated from thedinoflagellate Dinophysis acuta collected from the west coast of New Zealand. The structure of PTX11was determined as 34S-hydroxypectenotoxin-2 by tandem mass spectrometry and UV and NMRspectroscopy. PTX11 appears to be only the third pectenotoxin identified as a natural biosynthetic productfrom algae after pectenotoxin-2 and pectenotoxin-12. The LD50 of PTX11 determined by mouseintraperitoneal injection was 244 
g/kg. The LDmin of PTX11 in these experiments was 250 g/kg. Nosigns of toxicity were recorded in mice following an oral dose of PTX11 at 5000 g/kg. No diarrhea wasobserved in any of the animals administered with the test substance by either route of administration.Unlike pectenotoxin-2 (PTX2), PTX11 was not readily hydrolyzed to its corresponding seco acid byenzymes from homogenized green-lipped mussel (Perna canaliculus) hepatopancreas.
g/kg. The LDmin of PTX11 in these experiments was 250 g/kg. Nosigns of toxicity were recorded in mice following an oral dose of PTX11 at 5000 g/kg. No diarrhea wasobserved in any of the animals administered with the test substance by either route of administration.Unlike pectenotoxin-2 (PTX2), PTX11 was not readily hydrolyzed to its corresponding seco acid byenzymes from homogenized green-lipped mussel (Perna canaliculus) hepatopancreas.