Quantitative Determination of Tenuazonic Acid in Pig and Broiler Chicken Plasma by LC-MS/MS and Its Comparative Toxicokinetics

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• 作者：Sophie Fraeyman ; Mathias Devreese ; Nathan Broekaert ; Thomas De Mil ; Gunther Antonissen ; Siegrid De Baere ; Patrick De Backer ; Michael Rychlik ; Siska Croubels
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 30, 2015
• 年：2015
• 卷：63
• 期：38
• 页码：8560-8567
• 全文大小：331K
• ISSN：1520-5118

文摘

A liquid chromatography鈥搕andem mass spectrometry (LC-MS/MS) method to quantitate tenuazonic acid (TeA) in pig and broiler chicken plasma was successfully developed and validated. Linear matrix-matched calibration curves ranged between 5 and 200 ng/mL. Correlation coefficients, goodness-of-fit coefficients, and within-day and between-day precision and accuracy fell well within the acceptance criteria. The limit of quantitation was 5.0 ng/mL in both pig and broiler chicken plasma. The LC-MS/MS method was applied in a comparative toxicokinetic study in both pigs and broiler chickens. TeA was completely bioavailable after oral administration in both animal species. However, absorption was deemed to be slower in broiler chickens (mean t_max 0.32 h in pigs vs 2.60 h in chickens). TeA was more slowly eliminated in broiler chickens (mean t_1/2el 0.55 h in pigs vs 2.45 h in chickens after oral administration), mainly due to the significantly lower total body clearance (mean Cl 446.1 mL/h/kg in pigs vs 59.2 mL/h/kg in chickens after oral administration). Tissue residue studies and further research to elucidate the biotransformation and excretion processes of TeA in pigs, broiler chickens, and other animal species are imperative.