Association of a Specific Cationic Peroxidase Isozyme with Maize Stress and Disease Resistance Responses, Genetic Identification, and Identification of a cDNA Coding for the Isozyme

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• 作者：Patrick F. Dowd and Eric T. Johnson
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：June 1, 2005
• 年：2005
• 卷：53
• 期：11
• 页码：4464 - 4470
• 全文大小：172K
• 年卷期：v.53,no.11(June 1, 2005)
• ISSN：1520-5118

文摘

The presence of a pI 9.0 cationic peroxidase isozyme from milk stage pericarp of six susceptible andfive resistant inbreds was correlated significantly with previously reported field data on percentageinfection by Aspergillus flavus in the inbreds and their hybrids. The isozyme was constitutivelyexpressed in some additional maize tissues and lines examined, and frequently induced by mechanicaldamage, heat shock, Fusarium proliferatum, and/or Bacillus subtilis in other lines tested. Native/IEFtwo-dimensional electrophoresis identified the isozyme as the previously genetically identified px5.A cDNA clone expressed in black Mexican sweet (BMS) maize cell cultures produced the pI 9.0isozyme. In addition to potential use in marker-assisted breeding, enhanced expression of this cationicperoxidase through breeding or genetic engineering may lead to enhanced disease or insectresistance.Keywords: Maize; peroxidase; Fusarium; Aspergillus; disease resistance