Multicomponent Chemical Imaging of Pharmaceutical Solid Dosage Forms with Broadband CARS Microscopy

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• 作者：Christopher M. Hartshorn ; Young Jong Lee ; Charles H. Camp ; Jr. ; Zhen Liu ; John Heddleston ; Nicole Canfield ; Timothy A. Rhodes ; Angela R. Hight Walker ; Patrick J. Marsac ; Marcus T. Cicerone
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：September 3, 2013
• 年：2013
• 卷：85
• 期：17
• 页码：8102-8111
• 全文大小：571K
• 年卷期：v.85,no.17(September 3, 2013)
• ISSN：1520-6882

文摘

We compare a coherent Raman imaging modality, broadband coherent anti-Stokes Raman scattering (BCARS) microscopy, with spontaneous Raman microscopy for quantitative and qualitative assessment of multicomponent pharmaceuticals. Indomethacin was used as a model active pharmaceutical ingredient (API) and was analyzed in a tabulated solid dosage form, embedded within commonly used excipients. In comparison with wide-field spontaneous Raman chemical imaging, BCARS acquired images 10脳 faster, at higher spatiochemical resolution and with spectra of much higher SNR, eliminating the need for multivariate methods to identify chemical components. The significant increase in spatiochemical resolution allowed identification of an unanticipated API phase that was missed by the spontaneous wide-field method and bulk Raman spectroscopy. We confirmed the presence of the unanticipated API phase using confocal spontaneous Raman, which provided spatiochemical resolution similar to BCARS but at 100脳 slower acquisition times.