Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

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• 作者：Tania Kjellerup Lind ; Marit茅 C谩rdenas ; Hanna Pauliina Wacklin
• 刊名：Langmuir
• 出版年：2014
• 出版时间：July 1, 2014
• 年：2014
• 卷：30
• 期：25
• 页码：7259-7263
• 全文大小：284K
• ISSN：1520-5827

文摘

We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T_m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T_m by neutron reflection and investigated the effect of subsequent cooling to below the T_m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T_m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.