Nonenzymatic Transglycosylation Reactions Induced by Roasting: New Insights from Models Mimicking Coffee Bean Regions with Distinct Polysaccharide Composition

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• 作者：Ana S. P. Moreira ; Joana Simões ; Fernando M. Nunes ; Dmitry V. Evtuguin ; Pedro Domingues ; Manuel A. Coimbra ; M. Rosário M. Domingues
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：March 2, 2016
• 年：2016
• 卷：64
• 期：8
• 页码：1831-1840
• 全文大小：477K
• ISSN：1520-5118

文摘

Three mixtures containing different molar proportions of (β1→4)-d-mannotriose and (α1→5)-l-arabinotriose, oligosaccharides structurally related to coffee polysaccharides (galactomannans and arabinogalactans), were roasted at 200 °C for different periods. Electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MSⁿ) analyses of labeled (¹⁸O) and unlabeled samples allowed identification of not only nonhybrid oligosaccharides but also hybrid oligosaccharides composed of both hexose and pentose units. The identification of hybrid oligosaccharides allowed us to infer the occurrence of nonenzymatic transglycosylation reactions involving both oligosaccharides in the starting mixtures. Also, it was observed that using different proportions of the oligosaccharides in the starting mixtures and extents of thermal treatment led to a variation in the composition of the compounds formed. These results have led to the conclusion that, depending on the distribution of the polysaccharides in the bean cell walls and the roasting conditions, different nonhybrid and hybrid structures can be formed during coffee roasting.