Biochemical Characterization of a Eukaryotic Decalin-Forming Diels–Alderase
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- 作者：Li Li ; Peiyuan Yu ; Man-Cheng Tang ; Yi Zou ; Shu-Shan Gao ; Yiu-Sun Hung ; Muxun Zhao ; Kenji Watanabe ; K. N. Houk ; Yi Tang
- 刊名：Journal of the American Chemical Society
- 出版年：2016
- 出版时间：December 14, 2016
- 年：2016
- 卷：138
- 期：49
- 页码：15837-15840
- 全文大小：358K
- ISSN：1520-5126
文摘
The trans-decalin structure formed by intramolecular Diels–Alder cycloaddition is widely present among bioactive natural products isolated from fungi. We elucidated the concise three-enzyme biosynthetic pathway of the cytotoxic myceliothermophin and biochemically characterized the Diels–Alderase that catalyzes the formation of trans-decalin from an acyclic substrate. Computational studies of the reaction mechanism rationalize both the substrate and stereoselectivity of the enzyme.