Poly(L-lysine)-Graft-Chitosan Copolymers: Synthesis, Characterization, and Gene Transfection Effect
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- 作者：Haijun Yu ; Xuesi Chen ; Tiancheng Lu ; Jing Sun ; Huayu Tian ; Jun Hu ; Yu Wang ; Peibiao Zhang ; Xiabin Jing
- 刊名：Biomacromolecules
- 出版年：2007
- 出版时间：May 2007
- 年：2007
- 卷：8
- 期：5
- 页码：1425 - 1435
- 全文大小：996K
- 年卷期：v.8,no.5(May 2007)
- ISSN：1526-4602
文摘
Polypeptide/polysaccharide graft copolymers poly(L-lysine)-graft-chitosan (PLL-g-Chi) were prepared by ring-opening polymerization (ROP) of 
-benzoxycarbonyl L-lysine N-carboxyanhydrides (Z-L-lysine NCA) in thepresence of 6-O-triphenylmethyl chitosan. The PLL-g-Chi copolymers were thoroughly characterized by 1H NMR,13C NMR, Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). The number-averagedegree of polymerization of PLL grafted onto the chitosan backbone could be adjusted by controlling the feedratio of NCA to 6-O-triphenylmethyl chitosan. The particle size of the complexes formed from the copolymerand calf thymus DNA was measured by dynamic light scattering (DLS). It was found in the range of 120~340nm. The gel retardation electrophoresis showed that the PLL-g-Chi copolymers possessed better plasmid DNA-binding ability than chitosan. The gene transfection effect in HEK 293T cells of the copolymers was evaluated,and the results showed that the gene transfection ability of the copolymer was better than that of chitosan andwas dependent on the PLL grafting ratio. The PLL-g-Chi copolymers could be used as effective gene deliveryvectors.
-benzoxycarbonyl L-lysine N-carboxyanhydrides (Z-L-lysine NCA) in thepresence of 6-O-triphenylmethyl chitosan. The PLL-g-Chi copolymers were thoroughly characterized by 1H NMR,13C NMR, Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). The number-averagedegree of polymerization of PLL grafted onto the chitosan backbone could be adjusted by controlling the feedratio of NCA to 6-O-triphenylmethyl chitosan. The particle size of the complexes formed from the copolymerand calf thymus DNA was measured by dynamic light scattering (DLS). It was found in the range of 120~340nm. The gel retardation electrophoresis showed that the PLL-g-Chi copolymers possessed better plasmid DNA-binding ability than chitosan. The gene transfection effect in HEK 293T cells of the copolymers was evaluated,and the results showed that the gene transfection ability of the copolymer was better than that of chitosan andwas dependent on the PLL grafting ratio. The PLL-g-Chi copolymers could be used as effective gene deliveryvectors.