PLA-PEG-PLA and Its Electroactive Tetraaniline Copolymer as Multi-interactive Injectable Hydrogels for Tissue Engineering
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- 作者：Haitao Cui ; Jun Shao ; Yu Wang ; Peibiao Zhang ; Xuesi Chen ; Yen Wei
- 刊名：Biomacromolecules
- 出版年：2013
- 出版时间：June 10, 2013
- 年：2013
- 卷：14
- 期：6
- 页码：1904-1912
- 全文大小：479K
- 年卷期：v.14,no.6(June 10, 2013)
- ISSN：1526-4602
文摘
Injectable hydrogels have served as biomimic scaffolds that provide a three-dimensional (3D) structure for tissue engineering or carriers for cell encapsulation in the biomedical field. In this study, the injectable electroactive hydrogels (IEHs) were prepared by introducing electrical properties into the injectable materials. Carboxyl-capped tetraaniline (CTA) as functional group was coupled with enantiomeric polylactide鈥損oly(ethylene glycol)鈥損olylactide (PLA-PEG-PLA), and the electroactive hydrogels were obtained by mixing the enantiomeric copolymers of CTA-PLLA-PEG-PLLA-CTA and CTA-PDLA-PEG-PDLA-CTA aqueous solutions. ultraviolet鈥搗isible spectroscopy (UV鈥搗is) and cyclic voltammetry (CV) of the complex solution showed good electroactive properties. The gelation mechanism and intermolecular multi-interactions such as stereocomplextion, hydrogen bonding, and 蟺鈥撓€ stacking were studied by Fourier transform infrared spectroscopy (FT-IR), UV鈥搗is, and wide-angle X-ray diffraction (WAXD). Gelation properties of the complexes were also studied by rheometer. The encapsulated cells remained highly viable in the gel matrices, suggesting that the hydrogels have excellent cytocompatibility. After subcutaneous injection, the gels were formed in situ in the subcutaneous layer, and hematoxylin鈥揺osin (H&E) staining suggested acceptable biocompatibility of our materials in vivo. Moreover, these injectable materials, when treated with pulsed electrical stimuli, were shown to be functionally active and to accelerate the proliferation of encapsulated fibroblasts, cardiomyocytes, and osteoblasts. Hence, the IEHs possessing these excellent properties would be potentially used as in vivo materials for tissue engineering scaffold.