Kinetics of Enzymatic Degradation of High Molecular Weight Polysaccharides through a Nanopore: Experiments and Data-Modeling

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• 作者：Aziz Fennouri ; R茅gis Daniel ; Manuela Pastoriza-Gallego ; Lo茂c Auvray ; Juan Pelta ; Laurent Bacri
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：September 17, 2013
• 年：2013
• 卷：85
• 期：18
• 页码：8488-8492
• 全文大小：389K
• 年卷期：v.85,no.18(September 17, 2013)
• ISSN：1520-6882

文摘

The enzymatic degradation of long polysaccharide chains is monitored by nanopore detection. It follows a Michaelis鈥揗enten mechanism. We measure the corresponding kinetic constants at the single molecule level. The simulation results of the degradation process allowed one to account for the oligosaccharide size distribution detected by a nanopore.