Battery-Operated, Portable, and Flexible Air Microplasma Generation Device for Fabrication of Microfluidic Paper-Based Analytical Devices on Demand

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• 作者：Peng-Kai Kao ; Cheng-Che Hsu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：September 2, 2014
• 年：2014
• 卷：86
• 期：17
• 页码：8757-8762
• 全文大小：300K
• ISSN：1520-6882

文摘

A portable microplasma generation device (MGD) operated in ambient air is introduced for making a microfluidic paper-based analytical device (渭PAD) that serves as a primary healthcare platform. By utilizing a printed circuit board fabrication process, a flexible and lightweight MGD can be fabricated within 30 min with ultra low-cost. This MGD can be driven by a portable power supply (less than two pounds), which can be powered using 12 V-batteries or ac-dc converters. This MGD is used to perform maskless patterning of hydrophilic patterns with sub-millimeter spatial resolution on hydrophobic paper substrates with good pattern transfer fidelity. Using this MGD to fabricate 渭PADs is demonstrated. With a proper design of the MGD electrode geometry, 渭PADs with 500-渭m-wide flow channels can be fabricated within 1 min and with a cost of less than $USD 0.05/device. We then test the 渭PADs by performing quantitative colorimetric assay tests and establish a calibration curve for detection of glucose and nitrite. The results show a linear response to a glucose assay for 1鈥?0 mM and a nitrite assay for 0.1鈥? mM. The low cost, miniaturized, and portable MGD can be used to fabricate 渭PADs on demand, which is suitable for in-field diagnostic tests. We believe this concept brings impact to the field of biomedical analysis, environmental monitoring, and food safety survey.