Radical Stabilization Is Crucial in the Mechanism of Action of Lysine 5,6-Aminomutase: Role of Tyrosine-263伪 As Revealed by Electron Paramagnetic Resonance Spectroscopy

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• 作者：Yung-Han Chen ; Amarendra N. Maity ; Yu-Chiang Pan ; Perry A. Frey ; Shyue-Chu Ke
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：November 2, 2011
• 年：2011
• 卷：133
• 期：43
• 页码：17152-17155
• 全文大小：773K
• 年卷期：v.133,no.43(November 2, 2011)
• ISSN：1520-5126

文摘

Adenosylcobalamin- and pyridoxal-5鈥?phosphate-dependent lysine 5,6-aminomutase utilizes free radical intermediates to mediate 1,2-amino group rearrangement, during which an elusive high-energy aziridincarbinyl radical is proposed to be central in the mechanism of action. Understanding how the enzyme participates in stabilizing any of the radical intermediates is fundamentally significant. Y263F mutation abolished the enzymatic activity. With isotope-edited EPR methods, the roles of the Tyr263伪 residue in the putative active site are revealed. The Tyr263伪 residue stabilizes a radical intermediate, which most likely is the aziridincarbinyl radical, either by acting as a spin-relay device or serving as an anchor for the pyridine ring of pyridoxal-5鈥?phosphate through aromatic 蟺-stacking interactions during spin transfer. The Tyr263伪 residue also protects the radical intermediate from interception by molecular oxygen. This study supports the proposed reaction mechanism, including the aziridincarbinyl radical, which has eluded detection for more than two decades.