Identification of Glycoproteins in Human Cerebrospinal Fluid with a Complementary Proteomic Approach

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• 作者：Sheng Pan ; Yan Wang ; Joseph F. Quinn ; Elaine R. Peskind ; Dana Waichunas ; Jake T. Wimberger ; Jinghua Jin ; Jane G. Li ; David Zhu ; Catherine Pan ; Jing Zhang
• 刊名：Journal of Proteome Research
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：5
• 期：10
• 页码：2769 - 2779
• 全文大小：406K
• 年卷期：v.5,no.10(October 2006)
• ISSN：1535-3907

文摘

Biomarkers are pressingly needed to assist with the clinical diagnosis of neurodegenerative diseasesand/or the monitoring of disease progression. Glycoproteins are enriched in bodily fluids such as humancerebrospinal fluid (CSF), an ideal source for discovering biomarkers due to its proximity to the centralnervous system (CNS), and consequently can serve as diagnostic and/or therapeutic markers for CNSdiseases. We report here an in-depth identification of glycoproteins in human CSF using a complementary proteomic approach which integrated hydrazide chemistry and lectin affinity column forglycoprotein enrichment, followed by multidimensional chromatography separation and tandem massspectrometric analysis. Using stringent criteria, a total of 216 glycoproteins, including many low-abundance proteins, was identified with high confidence. Approximately one-third of these proteinswas already known to be relevant to the CNS structurally or functionally. This investigation, for thefirst time, not only categorized many glycoproteins in human CSF but also expanded the existing overallCSF protein database.