文摘
Crataegus laevigata and Crataegus monogyna (hawthorn) were subjected to drought and cold stresstreatments, and polyphenolic extracts from control and stress-treated plants were assayed forantioxidant capacities using a modified version of the Total Antioxidant Status Assay (Randox, SanFrancisco, CA). In addition, these plants were analyzed for levels of flavanol-type substance [(-)-epicatechin] and flavonoid (vitexin 2' '-O-rhamnoside, acetylvitexin 2' '-O-rhamnoside, and hyperoside)constituents that are important metabolites in hawthorn herbal preparations used to treat patientswith heart disease. Drought and cold stress treatments caused increases in levels of (-)-epicatechinand hyperoside in both Crataegus species. Such treatments also enhanced the antioxidant capacityof the extracts. The results from this study thus indicate that these kinds of stress treatments canenhance the levels of important secondary metabolites and their total antioxidant capacities in leavesof Crataegus.Keywords: Crataegus; hawthorn leaves; stress treatment; flavanols; flavonoids; antioxidant capacity