Elimination of Inhaled 3,3鈥?Dichlorobiphenyl and the Formation of the 4-Hydroxylated Metabolite

详细信息    查看全文

• 作者：Xin Hu ; Hans-Joachim Lehmler ; Andrea Adamcakova-Dodd ; Peter S. Thorne
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：47
• 期：9
• 页码：4743-4751
• 全文大小：388K
• 年卷期：v.47,no.9(May 7, 2013)
• ISSN：1520-5851

文摘

The recent discovery of 3,3鈥?dichlorobiphenyl (CB11) as a byproduct of pigment manufacturing underscores the urgency to investigate its biological fate. The high level and ubiquity of atmospheric CB11 indicates that inhalation is the major route of exposure. However, few data on its uptake and elimination exist. A time course study was performed exposing male Sprague鈥揇awley rats to CB11 via nose-only inhalation with necropsy at 0, 4, and 8 h post exposure. An analytical method for CB11 and monohydroxylated metabolites employing pressurized liquid extraction and gas chromatography鈥搈ass spectrometry yielded efficient recovery of CB11 (73 卤 9%) and its metabolite 3,3鈥?dichlorobiphenyl-4-ol (4-OH鈥揅B11) (82 卤 12%). Each rat was exposed to 106 渭g/m³ vapor-phase CB11 for 2 h and received an estimated dose of 1.8 渭g. Rapid apparent first-order elimination of CB11 was found in lung, serum, and liver with half-lives of 1.9, 1.8, and 2.1 h, respectively. 4-OH鈥揅B11 was detected in the liver but not the lung or serum of exposed animals and displayed apparent first-order elimination with a 2.4 h half-life. This study demonstrates rapid metabolism of CB11 and elimination of 4-OH鈥揅B11 and suggests that the metabolite is not retained in the body but is susceptible to further biotransformation.