Development and Characterization of a GC-Enabled QLT-Orbitrap for High-Resolution and High-Mass Accuracy GC/MS

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• 作者：Amelia C. Peterson ; Graeme C. McAlister ; Scott T. Quarmby ; Jens Griep-Raming ; Joshua J. Coon
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：October 15, 2010
• 年：2010
• 卷：82
• 期：20
• 页码：8618-8628
• 全文大小：430K
• 年卷期：v.82,no.20(October 15, 2010)
• ISSN：1520-6882

文摘

We detail the development and characterization of a GC/QLT-Orbitrap hybrid mass spectrometer capable of high resolution (up to 100000 at m/z 400) and sub-parts-per-million mass accuracy GC/MS. A high-duty cycle, innovative scan type, the nested scan, was implemented to synchronize the Orbitrap acquisition rate and the time scale of gas chromatography (up to 6.5 Hz at resolution 7500). We benchmark this instrument’s key figures of merit, including resolution, mass accuracy, linear dynamic range, and spectral accuracy, and demonstrate its performance for two challenging applications: the determination of polychlorinated dibenzo-p-dioxins (PCDD) and dibenzofurans (PCDF) in environmental samples and the profiling of primary metabolites in Arabidopsis thaliana extracts.