Structures of the O-Specific Polysaccharides from Yokenella regensburgei (Koserella trabulsii) Strains PCM 2476, 2477, 2478, and 2494: High-Resolution Magic-Angle Spinning NMR Investigat
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- 作者：Wojciech Jachymek ; Tomasz Niedziela ; Carl Petersson ; Czeslaw Lugowski ; Jolanta Czaja ; and Lennart Kenne
- 刊名：Biochemistry
- 出版年：1999
- 出版时间：September 7, 1999
- 年：1999
- 卷：38
- 期：36
- 页码：11788 - 11795
- 全文大小：184K
- 年卷期：v.38,no.36(September 7, 1999)
- ISSN：1520-4995
文摘
The structures of the carbohydrate O-specific side-chain moiety of the lipopolysaccharides(LPS) of Yokenella regensburgei, strains PCM 2476, 2477, 2478, and 2494, have been investigated by 1Hand 13C NMR, fast atom bombardment tandem mass spectrometry (FAB-MSMS), matrix-assisted laserdesorption ionization time-of-flight (MALDI-TOF) mass spectrometry, methylation analysis, partial acidhydrolysis, and immunological methods. It was concluded that the O-specific polysaccharides of strains2476, 2477, 2478, and 2494 are composed of the same basic trisaccharide repeating unit having the structure
3)-
-D-FucpNAc-(12)-L--D-Hepp-(13)-6-deoxy--L-Talp-(1, in which L--D-Hepp is L-glycero--D-manno-heptopyranose. The detailed analysis revealed, however, differences in O-acetylation patternsof the 6-deoxy-L-talose residue, with 2- and 4-O-acetyl disubstituted 3)-6-deoxy--L-Talp-(1 in strainPCM 2476 and a 2-O-acetylated residue in strains 2477, 2478, and 2494. These structures represent novel,trisaccharide repeating units of bacterial O-antigens that are characteristic and unique to the Y. regensburgeispecies. By use of the high-resolution magic-angle spinning (HR-MAS) technique, 1H NMR spectra ofthe O-polysaccharides directly in isolated LPS were obtained. This allowed for almost full assignmentand structural determination of the polysaccharide. By this technique the O-polysaccharide componentswere also observed in their original form directly on the surface of living bacterial cells.
3)--D-FucpNAc-(12)-L--D-Hepp-(13)-6-deoxy--L-Talp-(1, in which L--D-Hepp is L-glycero--D-manno-heptopyranose. The detailed analysis revealed, however, differences in O-acetylation patternsof the 6-deoxy-L-talose residue, with 2- and 4-O-acetyl disubstituted 3)-6-deoxy--L-Talp-(1 in strainPCM 2476 and a 2-O-acetylated residue in strains 2477, 2478, and 2494. These structures represent novel,trisaccharide repeating units of bacterial O-antigens that are characteristic and unique to the Y. regensburgeispecies. By use of the high-resolution magic-angle spinning (HR-MAS) technique, 1H NMR spectra ofthe O-polysaccharides directly in isolated LPS were obtained. This allowed for almost full assignmentand structural determination of the polysaccharide. By this technique the O-polysaccharide componentswere also observed in their original form directly on the surface of living bacterial cells.