Modeling Nanoparticle鈥揂lveolar Epithelial Cell Interactions under Breathing Conditions Using Captive Bubble Surfactometry

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• 作者：David Sch眉rch ; Dimitri Vanhecke ; Martin J. D. Clift ; David Raemy ; Dorleta Jimenez de Aberasturi ; Wolfgang J. Parak ; Peter Gehr ; Alke Petri-Fink ; Barbara Rothen-Rutishauser
• 刊名：Langmuir
• 出版年：2014
• 出版时间：May 6, 2014
• 年：2014
• 卷：30
• 期：17
• 页码：4924-4932
• 全文大小：403K
• 年卷期：v.30,no.17(May 6, 2014)
• ISSN：1520-5827

文摘

Many advances have been made in recent years in cell culture models of the epithelial barrier of the lung from simple monolayers to complex 3-D systems employing different cell types. However, the vast majority of these models still present a static air鈥搇iquid interface which is unrealistic given the dynamic nature of breathing. We present here a method where epithelial lung cells are integrated into a system, the captive bubble surfactometer, which allows the cyclical compression and expansion of the surfactant film at the air鈥搇iquid interface, thus modeling the dynamics of breathing. We found that cellular uptake of deposited gold nanoparticles was significantly increased under the dynamic (breathing) conditions of compression and expansion as compared to static conditions. The method could be very useful for studying nanoparticle鈥揳lveolar lung cell interactions under breathing conditions for applications in nanomedicine and toxicology.