Labeling of Steroids on Solid Phase
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- 作者：Jari Peuralahti ; Katriina Suonpä ; ä ; Kaj Blomberg ; Veli-Matti Mukkala ; and Jari Hovinen
- 刊名：Bioconjugate Chemistry
- 出版年：2004
- 出版时间：July 2004
- 年：2004
- 卷：15
- 期：4
- 页码：927 - 930
- 全文大小：64K
- 年卷期：v.15,no.4(July 2004)
- ISSN：1520-4812
文摘
Up to four tetra-tert-butyl-1-[4-aminoacetamido)benzyl]diethylenetriaminetetrakis(acetato) derivatives of Fmoc glutamic acid (1) were attached to two steroids (17
-hydroxyprogesterone-3-O-carboxymethyloxime 2 and 1,3,5(10)-estratriene-3,16,17
-triol-6-one-6-O-carboxymethyloxime, 3)) onsolid phase using an oligopeptide synthesizer. Upon deprotection and conversion to the correspondingeuropium(III) chelates, these steroid conjugates were used in DELFIA-based competitive fluoroimmunoassays. The more chelates conjugated to 17--hydroxyprogesterone, the more diluted antiserumcould be used in an immunoassay for 17--hydroxyprogesterone, without any alteration of themeasurement range. Hence, 17--hydroxyprogesterone tracers with several chelates are useful whena high serum dilution factor is desired i.e., when only a limited quantity of antiserum is available.The result demonstrates the suitability and usefulness of lanthanide(III) chelates as multilabels inbioaffinity assays.
-hydroxyprogesterone-3-O-carboxymethyloxime 2 and 1,3,5(10)-estratriene-3,16,17-triol-6-one-6-O-carboxymethyloxime, 3)) onsolid phase using an oligopeptide synthesizer. Upon deprotection and conversion to the correspondingeuropium(III) chelates, these steroid conjugates were used in DELFIA-based competitive fluoroimmunoassays. The more chelates conjugated to 17--hydroxyprogesterone, the more diluted antiserumcould be used in an immunoassay for 17--hydroxyprogesterone, without any alteration of themeasurement range. Hence, 17--hydroxyprogesterone tracers with several chelates are useful whena high serum dilution factor is desired i.e., when only a limited quantity of antiserum is available.The result demonstrates the suitability and usefulness of lanthanide(III) chelates as multilabels inbioaffinity assays.