Platycodon grandiflorum A. De Candolle Ethanolic Extract Inhibits Adipogenic Regulators in 3T3-L1 Cells and Induces Mitochondrial Biogenesis in Primary Brown Preadipocytes

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• 作者：Hye-Lin Kim ; Jinbong Park ; Hyewon Park ; Yunu Jung ; Dong-Hyun Youn ; JongWook Kang ; Mi-Young Jeong ; Jae-Young Um
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 9, 2015
• 年：2015
• 卷：63
• 期：35
• 页码：7721-7730
• 全文大小：457K
• ISSN：1520-5118

文摘

This study was designed to evaluate the effects of Platycodon grandiflorum A. DC. ethanolic extract (PG) on obesity in brown/white preadipocytes. The effect of PG on the differentiation and mitochondrial biogenesis of brown adipocytes is still not examined. An in vivo study showed that PG induced weight loss in mice with high-fat-diet-induced obesity. PG successfully suppressed the differentiation of 3T3-L1 cells by down-regulating cellular induction of the peroxisome proliferators activated receptor 纬 (PPAR纬), CCAAT enhancer binding protein 伪 (C/EBP伪), lipin-1, and adiponectin but increasing expression of silent mating type information regulation 2 homologue 1 (SIRT1) and the phosphorylation of AMP-activated protein kinase 伪 (AMPK伪). The effect of PG on the adipogenic factors was compared with that of its bioactive compound platycodin D. In addition, PG increased expressions of mitochondria-related genes, including uncoupling protein 1 (UCP1), peroxisome proliferator activated receptor-coactivator 1 伪 (PGC1伪), PR domain containing 16 (PRDM16), SIRT3, nuclear respiratory factor (NRF), and cytochrome C (CytC) in primary brown adipocytes. These results indicate that PG stimulates the differentiation of brown adipocytes through modulation of mitochondria-related genes and could offer clinical benefits as a supplement to treat obesity.