Probing Protein鈭扢embrane Interactions Using Solid Supported Membranes

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• 作者：Ann Junghans ; Chloe虂 Champagne ; Philippe Cayot ; Camille Loupiac ; Ingo Ko虉per
• 刊名：Langmuir
• 出版年：2011
• 出版时间：March 15, 2011
• 年：2011
• 卷：27
• 期：6
• 页码：2709-2716
• 全文大小：974K
• 年卷期：v.27,no.6(March 15, 2011)
• ISSN：1520-5827

文摘

Tethered bilayer lipid membranes have been used as a model system to mimic the interactions between the whey protein 尾-lactoglobulin and a lipid interface. The approach allowed for a detailed study of the lipid鈭抪rotein interactions, the results being of possible importance in food and cosmetic applications. For such applications, lipid鈭抪rotein interactions and the interfacial behavior are vital factors in controlling and manipulating process conditions such as emulsion stabilization and gelification. Lipid composition as well as the structural properties of the protein governed their interactions, which were probed by a combination of surface plasmon spectroscopy, neutron reflectivity, and electrochemical impedance spectroscopy. Comparison of results obtained using native and a partially unfolded protein indicated that the protein preferentially forms loosely packed layers at the lipid interface.