Poly-2-methyl-2-oxazoline: A Peptide-like Polymer for Protein-Repellent Surfaces

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• 作者：Rupert Konradi ; Bidhari Pidhatika ; Andreas Mü ; hlebach ; Marcus Textor
• 刊名：Langmuir
• 出版年：2008
• 出版时间：February 5, 2008
• 年：2008
• 卷：24
• 期：3
• 页码：613 - 616
• 全文大小：241K
• 年卷期：v.24,no.3(February 5, 2008)
• ISSN：1520-5827

文摘

Surface coatings with so-called protein-repellent or nonfouling polymers have become indispensable for thedevelopment of modern therapeutic and diagnostic medical devices such as biosensors, drug-delivery capsules, andbiomedical implants. Nowadays, poly(ethylene glycol) (PEG) is routinely used for these purposes. However, thereis increasing evidence that PEG has limited long-term stability, particularly in vivo. Here we investigate poly(2-methyl-2-oxazoline) (PMOXA) as a potential alternative polymer. We designed comb copolymers consisting of apolycationic poly(L-lysine) backbone and PMOXA side chains by analogy to precisely studied and highly protein-repellent PEG-based systems. Using optical waveguide lightmode spectroscopy, we quantitatively compare the in situself-assembly of the comb copolymers on negatively charged surfaces and the exposure of the formed monolayersto full human serum. We find that the PMOXA-based coatings with an optimal side-chain grafting density eliminateprotein adsorption to a level of <2 ng/cm²; that is, they quantitatively equal the protein-repellent properties of thebest PEG-based coatings.