Nematicidal Activity of Allylisothiocyanate from Horseradish (Armoracia rusticana) Roots against Meloidogyne incognita

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• 作者：Nadhem Aissani ; Paola Tedeschi ; Annalisa Maietti ; Vincenzo Brandolini ; Vincenzo Luigi Garau ; Pierluigi Caboni
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：May 22, 2013
• 年：2013
• 卷：61
• 期：20
• 页码：4723-4727
• 全文大小：211K
• 年卷期：v.61,no.20(May 22, 2013)
• ISSN：1520-5118

文摘

In recent years, there has been a great development in the search for new natural pesticides for crop protection aiming a partial or total replacement of currently used chemical nematicides. Glucosinolate breakdown products are volatile and are therefore good candidates for nematodes fumigants. In this article, the methanol-aqueous extract (1:1, w/v) of horseradish (Armoracia rusticana) fresh roots (MAH) was in vitro tested for nematicidal activity against second stage (J2) Meloidogyne incognita. The EC₅₀ of MAH after 3 days of J2 immersion in test solutions was 251 卤 46 mg/L. The chemical composition analysis of the extract carried out by the GC-MS technique showed that allylisothicyanate was the most abundant compound. This pure compound induced J2 paralysis with an EC₅₀ of 52.6 卤 45.6 and 6.6 卤 3.4 mg/L after 1 h and 3 days of incubation. The use of LC-MS/MS showed for the first time that horseradish root is rich in polyphenols. The study of isothiocyanate degradation in soil showed that allylisothiocyanate was the most quickly degradable compound (half-life <10 min), whereas no significant differences in half-life time were noted between degradation in regular and autoclaved soil.

Keywords:

root-knot nematodes; GC-MS; LC-MS/MS; natural nematicides; Armoracia rusticana; allylisothiocyanate; isothiocyanates degradation