Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods

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• 作者：Marc Vaï ; tilingom ; Hans Pijnenburg ; Franç ; ois Gendre ; and Pierre Brignon
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1999
• 出版时间：December 1999
• 年：1999
• 卷：47
• 期：12
• 页码：5261 - 5266
• 全文大小：241K
• 年卷期：v.47,no.12(December 1999)
• ISSN：1520-5118

文摘

A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176"(Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. Theuse of the ABI Prism 7700 sequence detection system allowed the determination of the amplifiedproduct accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in sucha way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitativePCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNAextraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in somerepresentative food products were quantified.Keywords: "Maximizer" maize "event 176"; "Roundup Ready" soybean; real-time quantitative PCR;GMO detection