A New Approach to the Synthesis of APTRA Indicators

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• 作者：Pieter A. Otten ; Robert E. London ; and Louis A. Levy
• 刊名：Bioconjugate Chemistry
• 出版年：2001
• 出版时间：January 2001
• 年：2001
• 卷：12
• 期：1
• 页码：76 - 83
• 全文大小：128K
• 年卷期：v.12,no.1(January 2001)
• ISSN：1520-4812

文摘

We have devised a general synthesis of Mg²⁺ indicators which is based on the aminophenol triaceticacid (APTRA) structure. The key step is a palladium-catalyzed coupling reaction of a precursor of theAPTRA ligand with a fluorescent group. This strategy resulted in new ratioable fluorescent APTRAindicators and the finding that the fluorescence response of these indicators is different for Mg²⁺ andCa²⁺ in some cases. We believe that this represents a generally useful approach for combiningfluorophore and chelator functionalities.