4-Oxo-4H-quinolizine-3-carboxylic Acids as Mg2+ Selective, Fluorescent Indicators

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• 作者：Pieter A. Otten ; Robert E. London ; and Louis A. Levy
• 刊名：Bioconjugate Chemistry
• 出版年：2001
• 出版时间：March 2001
• 年：2001
• 卷：12
• 期：2
• 页码：203 - 212
• 全文大小：177K
• 年卷期：v.12,no.2(March 2001)
• ISSN：1520-4812

文摘

In this paper, we present the synthesis of a new series of substituted 4-oxo-4H-quinolizine-3-carboxylicacids and the evaluation of their fluorescent response upon complexation with Mg²⁺ and Ca²⁺. Thishas led to the development of the first Mg²⁺-selective, ratioable fluorescent indicators. We found that4-oxo-4H-quinolizine-3-carboxylates are excellent fluorophores which show a strong fluorescentresponse to Mg²⁺ but not to Ca²; the latter metal ion often causing interference during Mg²⁺measurements with previously described indicators. The dissociation constants of the studiedfluorophores-around 1.0 mM-are of the same order of magnitude as is usually observed forintracellular Mg²⁺.