Approach to the Biosynthesis of Atisine-Type Diterpenoid Alkaloids

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• 作者：Pei-Ji Zhao ; Suo Gao ; Li-Ming Fan ; Jing-Lei Nie ; Hong-Ping He ; Ying Zeng ; Yue-Mao Shen ; Xiao-Jiang Hao*
• 刊名：Journal of Natural Products
• 出版年：2009
• 出版时间：April 24, 2009
• 年：2009
• 卷：72
• 期：4
• 页码：645-649
• 全文大小：183K
• 年卷期：v.72,no.4(April 24, 2009)
• ISSN：1520-6025

文摘

To determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro cultured plantlets and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. l-[2-¹³C,¹⁵N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as a biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that l-[2-¹³C,¹⁵N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that l-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.